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1、Abstract:Lettuce, namely, lettuce, application of various varieties, production in recent years are cultivated area expanded rapidly.Through the use of plant tissue culture technology, using the versatility of plant cells, tissues and cells lettuce on callus induction and observation in vitro shoot growth situation, through orthogonal experiment design, research containing different chemical elements and hormone of lettuce in vitro shoot ***.Compare lettuce in vitro shoot best combination hormone levels, this experiment using the medium science orthogonal design explant medium, the selection of the best for the basic research and production related to provide the reference.This experiment in America with lettuce leaves Lactuca sativa as test materials (var), media, Longifolia) olumes of AgNO3, BA remains for test, and the content of each factor setting 3 different medium level to the proliferation of coefficient for determination of index, through orthogonal design L9 (34), which is suitable for lettuce callus differentiation in vitro shoot medium formula 1/2MS + BA 1mg/L + 0.4 mg/L + AgNO3 0.2 mg/L olumes.。